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Showing posts from April, 2025

What is Real-Time PCR? Its process and applications.

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  The real-time PCR, A game changer after traditional PCR machine.  A real-time PCR quantify the DNA/RNA in a real time with each cycle it progress. So you don't need to wait for the end, like in traditional PCR. In this blog we will focus on the concept of real-time PCR, its process and its application.  What is it basically? Just imagine you have only one DNA fragement which has a very unique coding genes which is responsible for a causing particular disease. We need this fragment in a large quantity for the study purpose. Like in a traditional PCR we have to wait for last part of the PCR cycle to happen and then we will go for visualization but in that also we are not getting the exact copy number that we had apmlified.  To solve this problem Russel Higuchi and his team in 1992  developed real-time PCR.  In real-time PCR the amount of product formed is monitored during the course of the reaction by monitoring the fluorescence of dyes or probes intro...

🔬 Liquid Biopsy: The Future of Non-Invasive Diagnosis and Precision Medicine

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  Imagine diagnosing cancer, monitoring tumor progression, or predicting disease relapse without ever needing a scalpel. That’s the promise of liquid biopsy —a revolutionary diagnostic technique that's turning the tide in modern medicine. In this blog, we’ll break down what liquid biopsy is, why it matters, and how it’s being used across medicine, from cancer to brain diseases to organ transplantation. 💧 What is Liquid Biopsy? A liquid biopsy analyzes biomarkers in bodily fluids —typically blood, but also saliva, urine, and cerebrospinal fluid (CSF). Unlike traditional tissue biopsies, which require invasive procedures, liquid biopsies allow for real-time, non-invasive insight into disease processes . The main components analyzed include: Circulating tumor DNA (ctDNA) Cell-free DNA (cfDNA) Circulating tumor cells (CTCs) Exosomes and extracellular vesicles This method allows clinicians to monitor diseases more dynamically—especially cancers—providing better pat...

🧬 Biotech vs. Traditional Biology: What’s the Difference?

 ✍️ Introduction Biotechnology and biology—two terms often used interchangeably—are actually quite different. While both revolve around the study of life, they serve unique purposes and offer distinct contributions to science and society. In this post, we'll explore how traditional biology and biotechnology differ, and use real-world examples from recent innovations to show just how powerful biotechnology has become. 🌱 What is Traditional Biology? Biology is the natural science that studies life and living organisms. This includes: Their structure and function Growth and evolution Distribution and interaction with the environment Traditional biology is more about observation, discovery, and understanding . It lays the foundation for all life sciences. Think about: Dissecting frogs in school Studying ecosystems in environmental science Learning how cells divide or how genes are passed down Biologists typically focus on how nature works , without cha...

History of Biotechnology: From Bread to Biopharma

✍️ Introduction Biotechnology may seem like a product of high-tech labs and modern science, but its roots go deep into human history. From the first loaf of bread to cutting-edge gene therapies, the journey of biotechnology is a fascinating story of human curiosity and innovation. Let’s travel through time and explore how this incredible science has evolved! 🕰️ 1. Ancient Biotechnology (Before 1800s) Long before we understood cells and DNA, humans were already using natural biological processes to improve life. 7000 BCE : Ancient people discovered fermentation—using yeast to make bread, beer, and wine. This was biotechnology in action! 6000 BCE : Egyptians and Sumerians used selective breeding to grow better crops and raise stronger animals. 4000 BCE : Fermented foods like yogurt and cheese became staples in ancient diets, thanks to bacterial action. Even without microscopes or scientific knowledge, early humans unknowingly harnessed biological processes to shape c...

The Overview of Biotech Jorney from Start to End.

Start the Biotech Journey Ancient Biotechnology (Before 1800s) Biotechnology, though a modern-sounding word, actually began thousands of years ago with the earliest human civilizations. Around 7000 BCE, people unknowingly practiced biotechnology when they discovered that natural processes like fermentation could transform grains into bread, and fruits into beer and wine, using wild yeasts.  As human societies developed, around 6000 BCE, the Egyptians and Sumerians began experimenting with selective breeding, choosing the best plants and animals to cultivate for better yield, taste, or strength. By 4000 BCE, the art of fermenting milk to produce cheese and yogurt had emerged, showing early humans’ deepening understanding of nature’s invisible helpers—microorganisms—long before they could even see them.  These ancient practices laid the foundation for the incredible science we know today as biotechnology. Classical Biotechnology (1800s – Mid 1900s) As science blossomed in the ...