What is Real-Time PCR? Its process and applications.
The real-time PCR, A game changer after traditional PCR machine. A real-time PCR quantify the DNA/RNA in a real time with each cycle it progress. So you don't need to wait for the end, like in traditional PCR. In this blog we will focus on the concept of real-time PCR, its process and its application. What is it basically? Just imagine you have only one DNA fragement which has a very unique coding genes which is responsible for a causing particular disease. We need this fragment in a large quantity for the study purpose. Like in a traditional PCR we have to wait for last part of the PCR cycle to happen and then we will go for visualization but in that also we are not getting the exact copy number that we had apmlified. To solve this problem Russel Higuchi and his team in 1992 developed real-time PCR. In real-time PCR the amount of product formed is monitored during the course of the reaction by monitoring the fluorescence of dyes or probes intro...